Photosynthesis converts light energy to chemical energy (glucose) in plants, algae, and cyanobacteria, using $6CO_{2} + \12H_{2}O (\12H_{2}O confirmed by Van Niel) to produce glucose + \6O_{2} + \6H_{2}O. It occurs in chloroplasts: light reactions on thylakoid membranes (grana) and the Calvin cycle in the stroma. Chlorophyll a is the primary pigment with reaction centres P680 (PS II) and P700 (PS I); accessory pigments broaden the spectrum and transfer energy to Chl a. PS II (P680) acts first, splitting water (Hill reaction: \2H_{2}O→4H^{+}+4e^{-}+O_{2})andinitiatingtheZ−schemeelectrontransportchain.Non−cyclicphotophosphorylation(bothPSIandPSII)producesATP,NADPH,andO_{2};cyclicphotophosphorylation(PSIonly,instromalamellae)producesATPonly.ATPsynthesisoccursbychemiosmosisthroughCF_{0}−CF_{1},drivenbytheH^{+}gradientbetweenthethylakoidlumenandstroma.TheCalvincycle(CRR)uses18ATPand12NADPHtofix6CO_{2}(viaRuBisCO+RuBP)intooneglucose,throughcarboxylation(→3−PGA),reduction(→G3P),andregeneration(→RuBP)stages.C4plants(maize,sugarcane)useKranzanatomyandPEPcarboxylasetofixCO_{2}spatially(mesophyll→bundlesheath),eliminatingphotorespiration;CAMplants(cacti,Bryophyllum)usetemporalseparation(nightCO_{2}fixation,dayCalvincycle).PhotorespirationisRuBisCO′swastefuloxygenaseactivity,producingphosphoglycolateandconsumingATPwithoutgain,absentinC4/CAMplants.Blackman′slawstatesthatphotosynthesisrateislimitedbytheleastavailablefactor—lightintensity,CO_{2}$ concentration, or temperature.