Part of REP-02 — Human Reproduction

Worked Problem — Tracing an Oocyte's Life

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Problem

Trace the complete life history of an oocyte from its formation in fetal life to the production of a mature ovum, specifying the stage, ploidy, and arrest conditions at each step.

Solution

Step 1 — Fetal life (~weeks 10–20 of gestation)

  • Primordial germ cells migrate to the gonad → become oogonia (2n)
  • Oogonia divide by mitosis → millions of oogonia formed
  • Oogonia enter meiosis I → become primary oocytes (2n)
  • PRIMARY OOCYTES ARREST AT PROPHASE I
  • Surrounded by granulosa cells → primordial follicles formed
  • ~2 million primary oocytes at birth

Step 2 — From birth to puberty

  • Primary oocytes remain arrested at prophase I
  • Atresia reduces pool to ~60,000–80,000 by puberty

Step 3 — Each menstrual cycle (from puberty)

  • FSH from anterior pituitary stimulates a cohort of follicles
  • One dominant follicle grows to Graafian follicle
  • LH surge → primary oocyte resumes meiosis I
  • Meiosis I completes → secondary oocyte (n) + polar body I
  • Secondary oocyte ARRESTS AT METAPHASE II

Step 4 — Ovulation (~day 14)

  • Graafian follicle ruptures → secondary oocyte (n) released
  • Fimbriae collect it → it enters the fallopian tube

Step 5 — If fertilized (in the ampulla)

  • Sperm penetrates zona pellucida (acrosomal reaction)
  • Cortical reaction occurs (polyspermy block)
  • Ca2+Ca^{2+} wave → metaphase II arrest breaks
  • Meiosis II completes → mature ovum (n) + polar body II
  • Ovum nucleus (female pronucleus) + sperm nucleus (male pronucleus) → fuse → zygote (2n)

Answer Summary

Oogonium (2n) → Primary oocyte (2n, arrested Prophase I) → Secondary oocyte (n, arrested Metaphase II) → Mature ovum (n, only after sperm entry)

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