Temperature Summary:
| Step | Temperature | Event |
|---|---|---|
| Denaturation | 94-98°C | H-bonds broken; dsDNA → ssDNA |
| Annealing | 50-65°C | Primers bind complementary template |
| Extension | 72°C | Taq polymerase synthesizes new strand |
Part of BT-01 — Biotechnology: Principles & Processes
Mermaid Process: PCR Thermal Cycling
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