Fill in the Blanks:
- Griffith showed that ________ from heat-killed S-strain transformed R-strain bacteria.
- Avery proved that ________ (enzyme) destroyed the transforming principle, confirming it was DNA.
- Hershey and Chase used ________ to label DNA and ________ to label protein.
- The Watson-Crick model describes DNA as a ________ double helix.
- In DNA, Adenine forms ________ hydrogen bonds with Thymine.
- Chargaff's rules state that A = ________ and G = ________.
- The nucleosome octamer contains two copies each of ________, ________, ________, ________.
- Meselson-Stahl proved DNA replication is ________.
- ________ enzyme unwinds the double helix; ________ synthesizes RNA primers.
- Okazaki fragments are found on the ________ strand.
- RNA polymerase does ________ require a primer.
- The template strand is read ________ to ________ by RNA polymerase.
- mRNA is synthesized ________ to ________ by RNA polymerase.
- The three stop codons are ________, ________, and ________.
- In translation, the ________ site receives incoming aminoacyl-tRNA; the ________ site is where the growing chain is attached.
- The Lac operon inducer (actual molecule) is ________.
- The repressor (encoded by ________) binds the ________ to block transcription.
- Structural genes in the Lac operon: ________ encodes beta-galactosidase; ________ encodes permease.
- The human genome contains approximately ________ bp and ________ protein-coding genes.
- DNA fingerprinting uses ________ detected by ________ blotting.
Answer Key:
- Transforming principle (DNA)
- DNase
- ^32P; ^35S
- Right-handed antiparallel
- 2
- T; C
- H2A, H2B, H3, H4
- Semiconservative
- Helicase; Primase
- Lagging
- Does not
- 3'; 5'
- 5'; 3'
- UAA, UAG, UGA
- A-site; P-site
- Allolactose
- lacI; operator
- lacZ; lacY
- 3.2 billion; 20,000–25,000
- VNTRs; Southern