Part of GEN-03 — Molecular Basis of Inheritance

Fill in the Blanks — Self-Test (with Answer Key)

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Fill in the Blanks:

  1. Griffith showed that ________ from heat-killed S-strain transformed R-strain bacteria.
  2. Avery proved that ________ (enzyme) destroyed the transforming principle, confirming it was DNA.
  3. Hershey and Chase used ________ to label DNA and ________ to label protein.
  4. The Watson-Crick model describes DNA as a ________ double helix.
  5. In DNA, Adenine forms ________ hydrogen bonds with Thymine.
  6. Chargaff's rules state that A = ________ and G = ________.
  7. The nucleosome octamer contains two copies each of ________, ________, ________, ________.
  8. Meselson-Stahl proved DNA replication is ________.
  9. ________ enzyme unwinds the double helix; ________ synthesizes RNA primers.
  10. Okazaki fragments are found on the ________ strand.
  11. RNA polymerase does ________ doesdoesnot\frac{does}{does not} require a primer.
  12. The template strand is read ________ to ________ by RNA polymerase.
  13. mRNA is synthesized ________ to ________ by RNA polymerase.
  14. The three stop codons are ________, ________, and ________.
  15. In translation, the ________ site receives incoming aminoacyl-tRNA; the ________ site is where the growing chain is attached.
  16. The Lac operon inducer (actual molecule) is ________.
  17. The repressor (encoded by ________) binds the ________ to block transcription.
  18. Structural genes in the Lac operon: ________ encodes beta-galactosidase; ________ encodes permease.
  19. The human genome contains approximately ________ bp and ________ protein-coding genes.
  20. DNA fingerprinting uses ________ detected by ________ blotting.

Answer Key:

  1. Transforming principle (DNA)
  2. DNase
  3. ^32P; ^35S
  4. Right-handed antiparallel
  5. 2
  6. T; C
  7. H2A, H2B, H3, H4
  8. Semiconservative
  9. Helicase; Primase
  10. Lagging
  11. Does not
  12. 3'; 5'
  13. 5'; 3'
  14. UAA, UAG, UGA
  15. A-site; P-site
  16. Allolactose
  17. lacI; operator
  18. lacZ; lacY
  19. 3.2 billion; 20,000–25,000
  20. VNTRs; Southern

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