Part of REP-02 — Human Reproduction

Fertilization and Early Development — Application Note

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Timeline from Ovulation to Implantation

Time Post-FertilizationEvent
Day 0Ovulation; secondary oocyte in ampulla
Day 0–1Fertilization (ampulla): acrosomal reaction → sperm entry → cortical reaction → meiosis II completion → zygote
Day 1–2Cleavage: 2-cell → 4-cell (fallopian tube, moving toward uterus)
Day 4Morula (16-cell solid ball)
Day 5Blastocyst (trophoblast + ICM + blastocoel)
Day 5–6Zona pellucida hatching
Day 6–7Implantation in endometrium
Day 7–8Trophoblast secretes hCG (detectable ~10 days post-implantation)

Blastocyst Structure

  • Trophoblast (outer) → placenta + hCG secretion
  • Inner cell mass (ICM) → embryo
  • Blastocoel → fluid-filled internal cavity

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